During B.V.Sc (equivalent to DVM) / M.V.SC / Ph.D Program

None

K1. Diagnostic procedure

1. Conduct of physical examination
2. Clinical pathology (e.g. hematology, biochemistry, urinalysis, faecal examination and skin scrapings etc.)
3. Other diagnostic procedures (e.g. Imaging, Ultrasound etc.)

K2. Surgical techniques associated with diagnostic (e.g. exploratory biopsy), therapeutic purpose (e.g. tumor removal) and creation of animal models.

K3. Immunotherapy (e.g. antibody response; cellular immunity; species-specific immune response)

K4. Nutrition with emphasis on effects of deficiency or toxicity

At the time of certification

T1. Prevent spontaneous or unintended disease or condition

T2. Control spontaneous or unintended disease or condition

T3. Diagnose disease or condition as appropriate

T4. Treat disease or condition as appropriate

K5. Anatomy with emphasis on features which have significance with regard to clinical medicine (e.g. rat Harderian gland) or experimental medicine (e.g. coronary artery anatomy of the pig, which allows use for induced infarcts; Circle of Willis anatomy in gerbils, which allows use in stroke models).

K6. Physiology with emphasis on normative data and characteristics (e.g. seasonal changes in squirrel monkeys; coprophagia in rabbits), metabolic differences (e.g. hypoglycemia in squirrel monkeys) or metabolism of induced disease (e.g. streptozotocin-induced diabetes mellitus; bleomycin and other agents induced fibrosis), reproductive physiology, and clinically significant physiological features.

K7. Parasitology with emphasis on parasitic diseases that can become established in a colony and zoonotic parasitic diseases.

K8. Microbiology with emphasis on organisms of clinical significance, subclinical infections that cause physiologic, biochemical, and/or immunologic alterations, zoonotic disease organisms, organisms and experimentally to induce infection and unintended infections (e.g. infections associated with chronic vascular cannulation), and sampling and culture techniques for such organisms.

K9. Anatomic pathology including pathogenesis of significant naturally occurring (e.g. tuberculosis) and experimentally induced (e.g. collagen induced arthritis) diseases, typical gross and histopathologic lesions (e.g. age-related changes, adverse phenotypes associated with genetically modified rodents) and pertinent anatomic pathology techniques.

K10. Pharmacology with emphasis on drugs used to treat disease and drugs used to induce diseases which mimics human diseases (e.g., indications, use and contraindications of drugs; adverse reactions; adverse interactions; mechanisms of action; species specific toxicity), and drugs used to induce disease (e.g., azoxymethane to induce neoplasia, or Dextran sulfate sodium (DSS) to induce colitis

K11. Epidemiology including species-specific susceptibility to induced disease (e.g. modes of disease transmission, latency; persistence; prevalence; incidence).

K12. Preventive medicine (e.g. immunization; quarantine; prescreening tests)

K13. Diagnostic procedures

1. Species-specific behavioural assessment
2. Serologic, cytologic, and molecular diagnostic tests (e.g. PCR, ELISA, IFA, MFIA) and sampling techniques.

K14. Genetics with emphasis on control and treatment of naturally occurring and experimentally induced disease, predisposition to disease, and modes of inheritance.

During B.V.Sc (DVM) / M.V.Sc / Ph.D program

K1. Anatomy and physiology of pain and distress

K2. Patient monitoring

K3. Critical and post-procedural care techniques

K4. Methods of euthanasia

At the time of certification

T1. Recognize pain and/or distress

T2. Minimize or eliminate pain and/or distress

T3. Administration of anesthesia and assessment of stages

T4. Recommended Euthanasia procedures

K5. Assessment of pain and distress and measures to alleviate pain and suffering during the course of experimentation

K6. Causes of pain

K7. Causes of distress

K8. Effects of pain and distress on normative physiology and on research studies.

K9. Pharmacological interventions for pain and distress and their effects on physiology, including age and species differences for such interventions, and depth and duration of analgesia provided by such interventions.

K10. Nonpharmacological interventions for pain and distress and their effects on physiology, including age and species differences for such interventions.

K11. Humane endpoint criteria and euthanasia.

During B.V.Sc (DVM) / M.V.Sc / Ph.D program

None

K1. Principles of experimental design, statistics and research methodology

K2. Bio methodology techniques (e.g. collection of blood, body fluids, tissues, handling and restraint, administration of compounds and treatments)

K3. Animal models including normative biology relevant to the research (e.g. background lesions of common strains).

K4. Characterization of animal models (e.g. phenotyping, behavioral assessment)

K5. Genetics and nomenclature

At the time of certification

T1. Facilitate or provide research support

T2. Advise and consult with investigators on matters related to their research

T3. Design and conduct research

K6. Research methods and equipment (e.g. antibody production; adjuvants; tumor induction; imaging; data collection techniques such as telemetry; observation; behavioral assessment methods)

K7. Gnotobiotics

K8. Experimental surgical techniques and instrumentation

K9. Information resources

K10. Scientific writing

K11. 3R’s techniques (Replacement, Reduction and Refinement)

K12. Effective methods for communicating research-related concerns

K13. Aseptic requirements for performing surgery

K14. Genetic modification / engineering technology including application of molecular biology techniques (e.g. Humanized mice).

K15. Genomics, metabolomics, and proteomics

After certification

T1. Collaborate with other scientists on research projects

K16. Grant application, review and funding mechanisms

During a B.V.Sc (DVM) / M.V.Sc / Ph.D program

None

K1. Species-specific husbandry (e.g. nutrition, housing, exercise)

K2. Methods of sterilization, sanitation, and decontamination

K3. Animal identification systems

K4. Pest control (e.g. methods, hazards, and toxicity)

K5. Environmental monitoring

K6. Watering and feeding (e.g. liquid or restricted diets, ad libitum)

At the time of certification

T1. Develop animal husbandry programs with problem solving practices

T2. Manage or provide indirect management / oversight of animal husbandry programs

T3. Manage or provide indirect management / oversight of laboratory animal facilities

K7. Breeding colony management (e.g. systems and records, genetic monitoring).

K8. Environmental causes of physiological alterations in animals and their effects on research (e.g. sound, light, temperature, humidity, housing systems).

K9. Environmental enrichment, species-specific behavioral and psychological needs including creation and maintenance of appropriate social pairs or groups.

K10. Quality assurance techniques for animal care-related equipment (e.g. effectiveness of cage sanitation verification) and supplies (e.g. water, feed and bedding).

K11. Animal procurement considerations (e.g. vendor surveillance, genetic monitoring, transportation).

K12. Pathogen-free barriers (exclusion), maintenance of pathogen-free colonies via sentinel use and health surveillance programs

K13. Containment facilities

After certification

T4. Design laboratory animal facilities

K14. Selection-criteria for animal care-related equipment and supplies.

K15. Fiscal management as it relates to budgetary and financial issues associated with animal facility management (e.g. per diem rate setting; equipment cost comparisons).

K16. Human resource management as it relates to operation of animal care and use programs.

K17. Disaster planning

K18. Facility planning, design, and construction

K19. Mechanical, electrical and plumbing systems

K20. Waste management

K21. Housing systems (e.g. high density caging, aquatics)

During a B.V.Sc / M.V.Sc / Ph.D program

None

K1. Laws, regulations, policies and standards

K2. Animal welfare organizations, Animal Welfare Board of India - their role, functions and current status. Laws on animal welfare.

K3. Prevention of Cruelty to Animals (PCA) Act, 1960 {59 of 1960). Role and function of CPCSEA.

K4. Role of veterinarians in animal welfare, code of conduct and ethics.

At the time of certification

T1. Perform direct or delegated Attending Veterinarian responsibilities.

T2. Advocate for humane care and use of animals.

T3. Provide advice to occupational health and safety program

T4. Provide advise on biological, chemical and radiation hazards in an animal research program

T5. Serve as a member of IAEC (IACUC)

T6. Review protocols and provide advise to investigators and the IAEC (IACUC).

K5. Rules and regulations of CPCSEA.

K6. Role and function of the IAEC (IACUC)

K7. Protocols review including harm benefit analysis

K8.  Role and function of the Institutional Biosafety Committee (IBC).

K9. Role and function of AAALAC international

K10. Good Laboratory Practices (GLP)

K11. International laws, regulations, policies and standards.

K12. Guide for the Care and Use of Laboratory Animals (NRC).

K13. AVMA Guidelines for the Euthanasia of Animals (AVMA)

K14. Biosafety in Microbiological and Biomedical Laboratories (BMBL)

K15. Live Animals Regulations (IATA - International Air Transportation Association)

K16. Occupational Health and Safety in the Care and Use of Research Animals (ILAR / NRC).

K17. Responsible conduct of research

K18. Occupational Health and Safety in the Care and Use of Research Animals (ILAR / NRC).

K19.  Facility inspection and program review.

After certification

None

K19. International laws, policies, and standards of other countries or regions (e.g. USDA regulations; EU Directives; CCAC etc.)

During a B.V.Sc (DVM) / M.V.Sc / Ph.D program

None

K1. Philosophy and ethics of animal used, history and value of animal research

At the time of certification

T1. Train personnel in animal care and use

T2. Maintain current knowledge and continued competence in laboratory animal medicine

K2. Educational resources

K3. Certification programs

K4. Societal issues involving use of animals.

1. Organizations related to and/or supportive of laboratory animal medicine and animal research (e.g. LASA, AFLAS, AALAS, FELASA, ACLAM, JCLAM, ILAR, ICLAS, IACLAM etc.)
2. Organizations opposed to animal research (e.g. PETA, HSUS, AVA-Net, ALIVE) including their philosophy and opposition strategy.

After certification

T3. Provide education in academic and/or laboratory animal residency programs

T4. Outside of formal training programs, mentor those interested in or involving in laboratory animal medicine.

T5. Provide community outreach on animal care and use.

None

LASA India: Laboratory Animal Scientists Association, India;
CPCSEA: Committee for the Purpose of Control and Supervision of Experiments on Animals;
AALAS: American Association of Laboratory animal Science;
ILAR: Institute of Laboratory Animal Research;
AFLAS: Asian Federation of Laboratory Animal Science;
FELASA: Federation of European Laboratory Animal Science Association;
ACLAM: American College of Laboratory Animal Medicine;
ICLAM: College of Laboratory Animal Medicine;
IACLAM: International Association of Colleges of Laboratory Animal Medicine;
JCLAM: Japanese College of Laboratory Animal Medicine;
ILAR: Institute for Laboratory Animal Research;
CCAC: Canadian Council on Animal Care;
NRC: National Research Council;
PETA: People for the Ethical Treatment of Animals;
HSUS: Humane Society of the United States;
AVA-Net: Anti-vivisection Action Network;
ALIVE: ALL Life in a Viable Environment;
USDA: United States Department of Agriculture.

Primary Species

P1. Mouse (Mus musculus)
P2. Rat (Rattus norvegicus)
P3. Rabbit (Oryctolagus cuniculus)
P4. Guinea Pig (Cavea Porcellus)

Secondary Species

S1. Dog (Canis familiaris)
S2. Zebrafish (Danio rerio)
S3. Macaques (Macaca spp)
S4. Syrian hamster (Mesocricetus auratus)
S5. Goat (Capra hircus)
S6. Sheep (Ovis aries)
S7. Cat (Felis catus)

Tertiary Species

T1. Ferret (Mustela putorius furo)
T2. African clawed frog (Xenopus laevis and Xenopus tropicalis)
T3. Gerbil (Meriones spp.)
T4. Marmoset / tamarins (Callitrichidae)
T5. Squirrel monkey (Saimiri sciureus)
T6. Pig (Sus scrofa)
T7. Chicken (Gallus domestica)
T8. Pigeon (Columba livia)
T9. Horse (Equus caballus)
T10. Cattle (Bos Taurus)
T11. Other species including reptiles, birds and fish
T12. Invertebrates